Gencore - Sequencing

The Core currently processes samples primarily on the ABI Prism 3700 Automated DNA Analyzer for both Sequencing and Genotyping applications and also owns a Model 377 which is sometimes used for backup capacity. The 3700 is designed for high throughput runs and is capable of processing up to four 96-well plates a day as well as handling 384-well plate formats. The ABI 3700 Sequence Analyzer is a fully automated system that allows for the unattended analysis of multiple 96-lane runs. Samples are electrophoresed through a system of capillaries using a polymer matrix.

Sequencing runs are done on Tuesdays and Thursdays. Average turn around time is 2-4 days depending on the workload. 500-800 base length reads are expected from one sequencing reaction, these results may vary depending on the particular template. We require your DNA samples to be submitted in 200 ul PCR tubes. Total volume should always be 6 ul either for plasmid or PCR template. We need 50 ng of DNA per 1kb of your product and 2 pmol of the primer. The ABI 3700 Genetic Analyzer used for sequencing is highly sensitive so it is crucial that DNA samples are purified and free from residual salts, proteins, residual detergents, and RNA. All samples must be in water - NO buffers. We need duplicates of your samples, so that we have the possibility to repeat the sequencing reaction if we need to. We provide the following standard primers at no cost. Please check the sequence with those in your sample to ensure they are the same. Some companies have made changes without changing the name of the primer.

T7: 5'-TAA-TAC-GAC-TCA-CTA-TAG-GG-3'
T3: 5'-ATT-ACC-CCT-CAC-TAA-AG-3'
M13F: 5'-TGT-AAA-ACG-ACG-GCC-AGT-3'
M13R: 5'-CAG-GAA-ACA-GCT-ATG-AC-3'
PGEX3: 5'-CCG-GGA-GCT-GCA-TGT-GTC-AGA-GG-3'
PGEX5: 5'-GGG-CTG-GCA-AGC-CAC-GTT-TGG-TG-3'
SP6: 5'-ATT-TAG-GTG-ACA-CTA-TAG-A-3'

Sequence & run per sample, $10 (Starting September 1, 2005)
Run only, per plate - please inquire gencore@bu.edu

All samples must have duplicates at the time of the submission or re-runs will charged at the normal rate (not free).


Sequencing The Core currently processes samples primarily on the ABI Prism 3700 Automated DNA Analyzer for both Sequencing and Genotyping applications and also owns a Model 377 which is sometimes used for backup capacity. The 3700 is designed for high throughput runs and is capable of processing up to four 96-well plates a day as well as handling 384-well plate formats. The ABI 3700 Sequence Analyzer is a fully automated system that allows for the unattended analysis of multiple 96-lane runs. Samples are electrophoresed through a system of capillaries using a polymer matrix. Sequencing runs are done on Tuesdays and Thursdays. Average turn around time is 2-4 days depending on the workload. 500-800 base length reads are expected from one sequencing reaction, these results may vary depending on the particular template. We require your DNA samples to be submitted in 200 ul PCR tubes. Total volume should always be 6 ul either for plasmid or PCR template. We need 50 ng of DNA per 1kb of your product and 2 pmol of the primer. The ABI 3700 Genetic Analyzer used for sequencing is highly sensitive so it is crucial that DNA samples are purified and free from residual salts, proteins, residual detergents, and RNA. All samples must be in water - NO buffers. We need duplicates of your samples, so that we have the possibility to repeat the sequencing reaction if we need to. We provide the following standard primers at no cost. Please check the sequence with those in your sample to ensure they are the same. Some companies have made changes without changing the name of the primer. T7: 5'-TAA-TAC-GAC-TCA-CTA-TAG-GG-3' T3: 5'-ATT-ACC-CCT-CAC-TAA-AG-3' M13F: 5'-TGT-AAA-ACG-ACG-GCC-AGT-3' M13R: 5'-CAG-GAA-ACA-GCT-ATG-AC-3' PGEX3: 5'-CCG-GGA-GCT-GCA-TGT-GTC-AGA-GG-3' PGEX5: 5'-GGG-CTG-GCA-AGC-CAC-GTT-TGG-TG-3' SP6: 5'-ATT-TAG-GTG-ACA-CTA-TAG-A-3' Pricing Sequence & run per sample, $10 (Starting September 1, 2005) Run only, per plate - please inquire gencore@bu.edu Req. Form Download >> click here to download Policy Note All samples must have duplicates at the time of the submission or re-runs will charged at the normal rate (not free).
About \| People \| Research \| Events \| Courses \| Gencore \| Opportunities \| Technology \| News

		HOME \| SEARCH